After becoming known for her role as Maria Torres on the late 1990s soap opera Sunset Beach, Chambers played Taylor Jones on the MTV soap Spyder Games and Molly Conlan on CBS's As the World Turns.
Before beginning her television acting career, she studied at The Catholic University of America and performed with both the Royal Shakespeare Company (in England) and the Shenandoah Shakespeare Express (in Virginia, U.S.A.).
Her film credits include roles in Scam, Life 101, and Behind the Camera: The Unauthorized Story of Charlie's Angels.
She and her three siblings grew up in Alexandria, Virginia, as the children of a mathematician mother and a physicist father.
In late 2006, she replaced Susan Haskell as the character Marty Saybrooke on ABC's One Life to Live. The following year, she left the show, and Haskell was re-cast as Marty.